Journal: Journal of Translational Medicine

Article Title: ANGPTL3 is a novel HDL component that regulates HDL function

doi: 10.1186/s12967-024-05032-x

Figure Lengend Snippet: Correlation between ANGPTL3 levels in HDL and HDL function in db/m and db/db mice. A Body weight of db/m and db/db mice. B Intraperitoneal glucose tolerance tests (IPGTT) in db/m and db/db mice. C The ANGPTL3 levels in HDL in db/m and db/db mice. D The cholesterol efflux of HDL isolated from db/m and db/db mice. The association between ANGPTL3 in HDL and the percentage of cholesterol efflux in db/m ( E ) and db/db mice ( F ). The expression of ICAM-1 ( G ) and VCAM-1 ( H ) in endothelial cells exposed to TNF-α and pre-incubated with HDL isolated from db/m and db/db mice

Article Snippet: Cells were stained with FITC mouse anti-human VCAM-1 (1:100) and PE mouse anti-human ICAM-1 (1:100) (BD Biosciences) antibodies for 30 min on ice in the dark.

Techniques: Isolation, Expressing, Incubation

Journal: Journal of Translational Medicine

Article Title: ANGPTL3 is a novel HDL component that regulates HDL function

doi: 10.1186/s12967-024-05032-x

Figure Lengend Snippet: ANGPTL3 directly regulated HDL function in mice. The plasma ANGPTL3 levels ( A ) and HDL-ANGPTL3 levels ( B ) in db/db mice injected with AAV virus encoding vector control, ANGPTL3, shRNA-NC and shRNA-ANGPTL3 plasmid respectively. The expression of ANGPTL3 in liver tissues of db/db mice injected with AAV-Vector and AAV-ANGPTL3 ( C ), AAV-shRNA-NC and AAV-shRNA-ANGPTL3 ( D ). E The percentage of cholesterol efflux to HDL isolated from plasma in db/db mice injected with AAV-Vector, ANGPTL3, shRNA-NC and shRNA-ANGPTL3. F The expression of ICAM-1 in endothelial cells against HDL. HDL were isolated from plasma in db/db mice injected with AAV-Vector, ANGPTL3, shRNA-NC and shRNA-ANGPTL3. The endothelial cells were treated with HDL, then exposed to TNF-α. The ICAM-1 expression was analyzed by Flow CytoMetry

Article Snippet: Cells were stained with FITC mouse anti-human VCAM-1 (1:100) and PE mouse anti-human ICAM-1 (1:100) (BD Biosciences) antibodies for 30 min on ice in the dark.

Techniques: Injection, Virus, Plasmid Preparation, shRNA, Expressing, Isolation, Flow Cytometry

Journal: eLife

Article Title: Piezo1 mechanosensing regulates integrin-dependent chemotactic migration in human T cells

doi: 10.7554/eLife.91903

Figure Lengend Snippet:

Article Snippet: Peptide, recombinant protein , Recombinant Human ICAM-1 , Peprotech , Cat# 150–05 , 4 μg/ml.

Techniques: Transfection, Construct, Plasmid Preparation, Recombinant, Sequencing, Control, esiRNA, Software